Effect of Different Nuts Oil Consumption on Morphological Features and Some Biomarkers of Inflammation in Adjuvant-Induced Arthritis (AIA) Rat Model

Year Published: 2023


Applied Sciences


Samiyah K. Al-Shammari, Doha M. Al-Nouri, Shaista ArzooORCID and Laila Naif Al-Harbi


This study evaluated the protective effect of different dietary ω-6/ω-3 ratios in oils obtained from various nuts (walnut, peanut, cashew, and hazelnut) against morphological features and markers of inflammation on an adjuvant-induced arthritis rat model. Rheumatoid arthritis (RA) was induced via intradermal injection of heat-killed Mycobacterium tuberculosis. Five groups of rats with RA (n = 5) were randomly categorized as follows: control positive, walnut oil group, peanut oil group, cashew nut oil group, and hazelnut oil group. Another five healthy rats served as a normal non-arthritic (control) group. We assessed the therapeutic effects by measuring arthritis scores during the experiment and serum inflammatory markers at the end of the study.

Key Findings

Key Findings: The serum levels of the rheumatoid factor, TNF-α, IL-6, IL-1β, and PGE2, were significantly (p ≤ 0.05) reduced in all treatment groups. The daily consumption of nut oils ameliorates clinical and morphological abnormalities by inhibiting the inflammatory cells that produce inflammatory interleukins and eicosanoids.